{"title":"Cell Dissociation Reagents","description":"\u003cp\u003eEnzymatic and non-enzymatic reagents for gentle cell detachment, harvesting, passaging, and downstream laboratory applications.\u003c\/p\u003e","products":[{"product_id":"trypsin-solution-10x-05-1250-10x","title":"Trypsin Solution (10X), 0.5% (1:250, 10X)","description":"\u003cp\u003e\u003cstrong\u003eTrypsin-EDTA solution 0.5% (10X)\u003c\/strong\u003e does not contain phenol red and is a common reagent used in cell culture to detach adherent cells from the surface of culture vessels. This solution contains trypsin, an enzyme that breaks down cell-cell and cell-substrate adhesions by cleaving proteins, and ethylenediaminetetraacetic acid (EDTA), which helps neutralize trypsin activity and prevent cell clumping by chelating calcium and magnesium ions. The absence of phenol red in this solution makes it suitable for cells sensitive to phenol red or when phenol red may interfere with specific assays or experiments. Phenol red is a pH indicator commonly found in cell culture media.\u003c\/p\u003e\n\u003cp\u003eBy using Trypsin-EDTA solution 0.5% (10X), researchers can effectively detach cells for passaging or experimentation while minimizing potential interference from phenol red, particularly when working with sensitive cell types or conducting assays where phenol red may affect the results.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eDisclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.\u003c\/em\u003e\u003c\/p\u003e","brand":"Plant Cell Technology","offers":[{"title":"PET \/ 100 mL","offer_id":46616504729697,"sku":"TRL02-100ML","price":26.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0598\/4973\/6289\/files\/square-pet-bottle.png?v=1790177103"},{"product_id":"trypsin-solution-025-1250-and-1mm-edta","title":"Trypsin Solution, 0.25% (1:250) and 1mM EDTA","description":"\u003cp\u003e\u003cstrong\u003eTrypsin-EDTA Solution (1X)\u003c\/strong\u003e, 0.25% Trypsin (1:250) and 1 mM EDTA in Hank's Balanced Salt Solution. Without Calcium and Magnesium.\u0026amp;nbsp;Porcine Parvovirus and Mycoplasma Tested. Shipped frozen, is used in tissue culture to detach adherent mammalian cells from the culture vessel by breaking down cell-cell and cell-substrate interactions. Trypsin is a proteolytic enzyme that cleaves peptide bonds, while EDTA inactivates calcium and magnesium ions needed for cell adhesion. This process aids in passaging, subculturing, and harvesting cells for downstream assays. Optimal concentration and exposure time must be determined for different cell types to maintain cell viability. After detachment, trypsin activity from Trypsin-EDTA Solution (1X) is halted by adding a medium and serum combination to protect the cells.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eDisclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.\u003c\/em\u003e\u003c\/p\u003e","brand":"Plant Cell Technology","offers":[{"title":"PET \/ 100 mL","offer_id":48922822344801,"sku":"TRL01-100ML","price":23.99,"currency_code":"USD","in_stock":true},{"title":"PET \/ 6X 100 mL","offer_id":48922822377569,"sku":"TRL01-6X100ML","price":129.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0598\/4973\/6289\/files\/square-pet-bottle.png?v=1790177103"}],"url":"https:\/\/pctlifesciences.com\/collections\/cell-dissociation-reagents.oembed","provider":"PCT Life Sciences","version":"1.0","type":"link"}